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Image Search Results
Journal: Nature Communications
Article Title: Neutrophil stunning by metoprolol reduces infarct size
doi: 10.1038/ncomms14780
Figure Lengend Snippet: ( a ) Experimental scheme and representative confocal images evaluating the effect of metoprolol on the number of co-aggregates of neutrophils (LysM-GFP+, green) and platelets (CD41, red) in the post-reperfused mouse myocardium. ( b , c ) LysM-GFP+ cells (neutrophils) attached to coronary vessels ( n =7–9) and the numbers of interacting platelets (CD41+) per neutrophil in reperfused myocardium. Scale bar, 20 μm. ( d ) Protocol scheme for the IR experiment evaluating the effect of neutrophil-platelet blockade with anti- PSGL1 Ab (administered 15 min after ischaemia onset, that is, 30 min before reperfusion) on the infarct-limiting effect of metoprolol (administered 35 min after ischaemia onset, that is,10 min before reperfusion). ( e – g ) AAR, infarct size, and representative images of Evans blue and TTC staining in vehicle- and metoprolol-treated mice pretreated with anti- PSGL1 Ab. n =5–7. NS, stands for non-significant. Data are means±s.e.m. * P <0.05; ** P <0.01. Comparison was determined by the nonparametric Wilcoxon–Mann–Whitney test.
Article Snippet: Dylight-650-conjugated anti-1A8 Ly6G (BE0075-1) and
Techniques: Staining, MANN-WHITNEY